Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Algorithm-Based Care for Treating Chronic Pancreatitis: A Nationwide Stepped-Wedge Cluster Randomized Controlled Trial.

The American journal of gastroenterology·2026
Same author

Active Matrixmetalloproteinase-8 in Periodontal Diagnosis: A Scoping Review.

Diagnostics (Basel, Switzerland)·2025
Same author

Unusual co-occurrence of cheilitis granulomatosa and plasma cell gingivitis: A case report.

Clinical advances in periodontics·2025
Same author

Combining oral fluid aMMP-8, calprotectin, and CCAAs in dental panoramic radiography for periodontal disease and systemic disease risk assessment: a point-of-care diagnostic approach.

Expert review of molecular diagnostics·2025
Same author

Reply to Markaryan et al. Comment on "Aji et al. aMMP-8 POCT vs. Other Potential Biomarkers in Chair-Side Diagnostics and Treatment Monitoring of Severe Periodontitis. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 9421".

International journal of molecular sciences·2025
Same author

Determination of the Stage of Periodontitis with 20 ng/mL Cut-Off aMMP-8 Mouth Rinse Test and Polynomial Functions in a Mobile Application.

Diagnostics (Basel, Switzerland)·2025

Related Experiment Video

Updated: Dec 2, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

5.0K

SARS-CoV-2 Detection in Gingival Crevicular Fluid.

S Gupta1, R Mohindra2, P K Chauhan3

  • 1Unit of Periodontics, Oral Health Sciences Centre, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.

Journal of Dental Research
|November 3, 2020
PubMed
Summary

Gingival crevicular fluid (GCF) can detect SARS-CoV-2, similar to saliva, offering insights into COVID-19 oral health connections. This study found GCF is a viable diagnostic fluid for severe acute respiratory syndrome coronavirus 2.

Keywords:
COVID-19diagnosticsoral healthoral hygieneperiodontalsaliva

More Related Videos

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
08:26

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Published on: June 23, 2022

1.9K
Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
11:11

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Published on: February 11, 2022

4.9K

Related Experiment Videos

Last Updated: Dec 2, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

5.0K
Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
08:26

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Published on: June 23, 2022

1.9K
Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
11:11

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Published on: February 11, 2022

4.9K

Area of Science:

  • Virology
  • Oral Medicine
  • Infectious Diseases

Background:

  • Understanding COVID-19 pathophysiology is crucial.
  • Gingival crevicular fluid (GCF) reflects systemic health and can detect viruses.
  • SARS-CoV-2 RNA detection in GCF is unexplored.

Purpose of the Study:

  • To assess GCF for SARS-CoV-2 presence in COVID-19 patients.
  • To compare GCF sensitivity with saliva for SARS-CoV-2 detection.

Main Methods:

  • Collected GCF and saliva from 33 COVID-19 positive patients.
  • Extracted total RNA and performed RT-qPCR for SARS-CoV-2 E gene.
  • Used nasopharyngeal swabs as the gold standard.

Main Results:

  • SARS-CoV-2 RNA was detected in GCF of a majority of patients.
  • GCF sensitivity was 63.64% and saliva sensitivity was 64.52%.
  • GCF and saliva showed comparable sensitivity for SARS-CoV-2 detection.

Conclusions:

  • GCF is a potential diagnostic fluid for SARS-CoV-2.
  • GCF may represent a possible transmission route for SARS-CoV-2.
  • This study provides evidence linking COVID-19 infection and oral health.