Developing a pathway-independent and full-autonomous global resource allocation strategy to dynamically switching
Junjun Wu1, Meijiao Bao2, Xuguo Duan3
1College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. wujunjun@njau.edu.cn.
Nature Communications
|November 3, 2020
Summary
This study introduces a novel dynamic resource allocation strategy for biological chemical production. It enables independent control of cellular resources for synthetic circuits, eliminating the need for human supervision during fermentation.
Area of Science:
- Synthetic Biology
- Metabolic Engineering
- Biochemical Production
Background:
- Cellular resources are limited, creating competition between synthetic circuits and host genes in biological chemical production.
- Current dynamic regulations for metabolic flux often rely on single quorum sensing (QS) circuits and offer localized control.
Purpose of the Study:
- To develop a pathway-independent dynamic resource allocation strategy for biological chemical production.
- To enable independent control over multiple cellular phenotypes, specifically growth and production.
Main Methods:
- Engineering synthetic orthogonal quorum-related circuits.
- Utilizing global mRNA decay mechanisms.
- Implementing a pathway-independent dynamic resource allocation strategy.
Main Results:
- Successfully achieved independent control of two distinct cellular phenotypic states.
- Enabled global redistribution of cellular resources towards synthetic circuits.
- Demonstrated a strategy for self-regulation of growth and production phenotypes.
Conclusions:
- The developed strategy offers pathway-independent and global self-regulation of cellular phenotypes.
- This approach significantly reduces or eliminates the need for human supervision in fermentation processes.
Related Concept Videos
01:22Diversity in Cell Signaling Responses
7.4K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity.
Graded and Abrupt Responses
Some signaling systems generate...
Graded and Abrupt Responses
Some signaling systems generate...
7.4K
01:23Operon Model
723
The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
723
01:28Global Regulatory Systems
385
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
385
01:24Other Glycolytic Pathways
585
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
585
01:16Transduction
794
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
794
01:19Interactions Between Signaling Pathways
7.0K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.0K