In Vivo Imaging with Genetically Encoded Redox Biosensors

Area of Science:

• Biochemistry
• Molecular Biology
• Physiology

Background:

• Redox reactions are crucial in physiology and disease pathogenesis.
• Analytical approaches in redox research are challenging due to unstable analyte nature.
• Genetically encoded sensors offer a novel solution for real-time redox measurements.

Purpose of the Study:

• To review the application of genetically encoded redox sensors in vivo.
• To summarize model systems and biological results from studies using these sensors.
• To highlight the capabilities of redox sensors for non-invasive biological pathway investigation.

Main Methods:

• Description of genetically encoded redox sensors used in vivo.
• Summarization of model systems applied in redox biology studies.
• Discussion of biological outcomes across various organisms.

Main Results:

• Genetically encoded sensors facilitate real-time registration of reactive molecules.
• Application in diverse model systems including animals, plants, and microorganisms.
• Enabled non-invasive investigation of in vivo biochemical pathways.

Conclusions:

• Genetically encoded sensors represent a powerful tool in redox biology.
• This technology advances the non-invasive study of redox processes in vivo.
• The review serves as a guide for researchers in redox biology and chemistry.