Related Experiment Video
Updated: Dec 2, 2025
Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
Published on: December 13, 2018
Long Non-Coding RNA NKILA Reduces Oral Squamous Cell Carcinoma Development Through the NF-KappaB Signaling Pathway
Daoyong Hu1, Tian Zhong2, Qun Dai1
1Department of General Dentistry, 47861The Affiliated Stomatological Hospital of Nanchang University and The Key Laboratory of Oral Biomedicine, Jiangxi Province, People's Republic of China.
Objective:
Emerging studies have identified that long non-coding RNAs (lncRNAs) play critical roles in cancer development. This study aims to explore the mechanism of NF-KappaB (NF-κB) interacting lncRNA (NKILA) in the pathological process of oral squamous cell carcinoma (OSCC).
Methods:
NKILA expression in OSCC tissues, paracancerous tissues, and normal human oral keratinocytes and OSCC cell lines was detected using RT-qPCR. KB cells were selected for the follow-up experiments. The role of NKILA in cell proliferation, migration, invasion, and NF-κB signaling pathway was identified using the gain- and loss-of function of NKILA in OSCC cells. Additionally, the role of NKILA in vitro was determined by inducing xenograft tumors in nude mice.
Results:
NKILA was poorly expressed in OSCC tissues and cells. Cell proliferation, invasion and migration, tumor volume and weight were significantly suppressed in cells with overexpressed NKILA, while silencing NKILA led to opposite trends. Moreover, the protein levels of p-IκBα and nuclear-p65 were markedly decreased, while the levels of IκBα and cytoplasm-p65 were enhanced in cells with overexpressed NKILA.
Conclusion:
This study provided evidence that NKILA could reduce proliferation, invasion and migration of OSCC cells through inhibiting the NF-κB signaling pathway. The findings may offer new insights for OSCC prevention and treatment.
Insights
Long non-coding RNA NKILA suppresses oral squamous cell carcinoma (OSCC) progression by inhibiting the NF-KappaB (NF-κB) pathway. This discovery offers potential new strategies for OSCC treatment and prevention.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.
- The specific mechanisms of lncRNAs in oral squamous cell carcinoma (OSCC) are under active investigation.
Purpose of the Study:
- To investigate the functional role and mechanism of the NF-KappaB (NF-κB) interacting lncRNA (NKILA) in oral squamous cell carcinoma (OSCC).
Main Methods:
- RT-qPCR was used to assess NKILA expression in OSCC tissues and cell lines.
- Gain- and loss-of-function experiments in OSCC cells evaluated NKILA's impact on proliferation, migration, and invasion.
- NF-κB signaling pathway activation was analyzed.
- In vivo studies involved xenograft tumor induction in nude mice.
Main Results:
- NKILA expression was found to be significantly lower in OSCC tissues and cells.
- Overexpression of NKILA suppressed OSCC cell proliferation, invasion, and migration, and reduced tumor growth in vivo.
- NKILA overexpression led to decreased p-IκBα and nuclear-p65, and increased IκBα and cytoplasmic-p65, indicating NF-κB pathway inhibition.
Conclusions:
- NKILA acts as a tumor suppressor in OSCC by inhibiting the NF-κB signaling pathway.
- These findings suggest NKILA as a potential therapeutic target for OSCC.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
02:39lncRNA - Long Non-coding RNAs
02:26NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...
02:15Experimental RNAi
02:30siRNA - Small Interfering RNAs
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
01:22MicroRNAs