Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

885
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
885
Photoluminescence: Applications01:14

Photoluminescence: Applications

833
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
833
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

12.5K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
12.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

European Cancer Prevention Organization on cancer screening: an expert panel position paper.

European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)·2026
Same author

Segmental Arterial Mediolysis Associated with Renal Allograft Artery Dissection and Thrombosis During Kidney Transplantation.

Clinics and practice·2026
Same author

Rethinking health technology assessment in robotic surgery: an EFISDS-TROGSS position paper. Official position paper of the European Federation - International Society for Digestive Surgery (EFISDS) and The Robotic Global Surgical Society (TROGSS).

Journal of robotic surgery·2026
Same author

Does Chronological Age Adequately Stratify Perioperative Risk? A Prospective Multicenter Cohort Study Using Frailty and Handgrip Strength.

Journal of clinical medicine·2026
Same author

Tailored Surgical Treatment and Outcomes in Solid Pseudopapillary Neoplasms of the Pancreas: A Case Series of Five Consecutive Paradigmatic Cases.

Diseases (Basel, Switzerland)·2026
Same author

Commentary on "Shifting Paradigm: Utilization and Outcomes with Neoadjuvant Chemotherapy for cT4 and cN2 Colon Cancers".

Cancers·2026

Related Experiment Video

Updated: Dec 2, 2025

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
08:23

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

13.3K

Methylene Blue-Current Knowledge, Fluorescent Properties, and Its Future Use.

Tomasz Cwalinski1, Wojciech Polom2, Luigi Marano3

  • 1Department of Surgical Oncology, Medical University of Gdansk, 80-214 Gdansk, Poland.

Journal of Clinical Medicine
|November 5, 2020
PubMed
Summary

Methylene blue, a long-used dye, is emerging as a valuable fluorophore for intraoperative imaging in surgery. Further research is needed to fully understand its potential and limitations in various surgical applications.

Keywords:
fluorescencefluorophoremethylene blue

More Related Videos

Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
05:59

Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

Published on: May 2, 2015

14.5K
Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
11:28

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

10.7K

Related Experiment Videos

Last Updated: Dec 2, 2025

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
08:23

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

13.3K
Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
05:59

Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

Published on: May 2, 2015

14.5K
Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
11:28

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

10.7K

Area of Science:

  • Medical Imaging
  • Surgical Technology
  • Fluorescent Dyes

Background:

  • Methylene blue, discovered in 1876, has diverse scientific applications.
  • Its recent adoption for intraoperative fluorescent imaging marks a new phase in its medical utility.

Purpose of the Study:

  • To review the evolving role of methylene blue as both a dye and a fluorophore in surgical settings.
  • To explore its potential, challenges, and limitations across specific surgical techniques.

Main Methods:

  • A comprehensive literature review of PubMed and Medline databases.
  • Analysis focused on historical, current, and future medical applications of methylene blue.
  • Investigation of its fluorophore function in five key surgical domains.

Main Results:

  • Methylene blue shows promise as a fluorescent agent in surgical visualization.
  • Applications include near-infrared imaging of ureters, parathyroid identification, pancreatic tumor imaging, and breast cancer margin detection/sentinel node biopsy.
  • It is used in many clinical procedures with a generally low patient risk profile.

Conclusions:

  • Methylene blue's use as a surgical fluorophore is in its early stages.
  • Extensive pre-clinical and clinical research is essential to fully elucidate its capabilities and constraints.
  • Continued investigation will optimize its application in various surgical interventions.