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Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force
Simona Capossela1, Vikas Mathew1, Manuela Boos2
1SCI Biobanking and Translational Medicine, Swiss Paraplegic Research, Nottwil, Switzerland.
Drug Design, Development and Therapy
|November 5, 2020
Summary
A novel shear force method offers a faster, easier, and more reproducible way to produce nano-erythrosomes (NEs) compared to traditional extrusion. This new technique ensures sterility and produces stable NEs, paving the way for large-scale production.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery Systems
Background:
- Conventional nano-erythrosome (NE) production via extrusion faces challenges including complex equipment, lengthy procedures, inconsistent pressure, and sterility concerns.
- Alternative methods like ultrasound probe sonication have shown limitations such as sample overheating and suboptimal NE characteristics compared to extrusion.
Purpose of the Study:
- To propose, develop, and evaluate a novel shear force-based method for nano-erythrosome (NE) fabrication.
- To compare the efficacy and characteristics of NEs produced by the new shear force method against the established extrusion technique.
Main Methods:
- Employed mechanical shear force using a rotor-stator tissue homogenizer to disrupt hemoglobin-depleted erythrocyte ghost membranes.
- Utilized identical batches of erythrocyte ghost membranes to directly compare shear force-produced NEs with extrusion-produced NEs.
- Characterized NEs for yield, size, encapsulation efficiency, morphology, and stability using flow cytometry, transmission electron microscopy, and zeta potential analysis.
Main Results:
- The shear force method demonstrated enhanced ease of setup, significantly reduced procedure time, improved sterility control, and decreased batch-to-batch variability.
- Shear force-generated NEs exhibited a desirable size distribution (~125 nm) and were morphologically and functionally equivalent to extrusion-derived NEs.
- NEs produced by shear force displayed excellent stability (counts, size, fluorescence) for 3 weeks at +4°C, along with good colloidal stability and resistance to stress.
Conclusions:
- The novel shear force method provides a faster, simpler, and highly reproducible approach for NE production compared to extrusion.
- This method facilitates simultaneous sterile batch production of NEs with homogenous size, enhanced stability, and improved shelf-life.
- The shear force technique's capacity for high-concentration sample processing suggests significant potential for future large-scale NE production and industrial applications.
