Nitric oxide and hydrogen sulfide protect plasma membrane integrity and mitigate chromium-induced methylglyoxal...

The present study aims to analyse the potential crosstalk between nitric oxide (NO) and hydrogen sulfide (H₂S) in triggering resilience of maize (Zea mays L.) seedlings to hexavalent chromium (Cr VI). Exogenous application of 500 μM sodium nitroprusside (SNP, as a NO donor) or sodium hydrosulfide (NaHS, as a H₂S donor) to 9-day-old maize seedlings, countered a Cr (200 μM) -elicited reduction in embryonic axis biomass. Cr caused cellular membrane injury by enhancing the levels of superoxide and hydroxyl radicals as well as methylglyoxal, and 4-hydroxy-2-nonenal. The application of SNP or NaHS considerably improved the endogenous NO and H₂S pool, decreased oxidative stress and lipid peroxidation by suppressing lipoxygenase activity and improving some antioxidant enzymes activities in radicles and epicotyls. Radicles were more affected than epicotyls by Cr-stress with enhanced electrolyte leakage and decreased proton extrusion as indicated by lesser H⁺-ATPase activity. H₂S appeared to mitigate Cr toxicity through up-regulated H⁺-ATPase and glyoxalase pathways and by maintaining optimal GSH levels as downstream effects of ROS and MG suppression. Hence, H₂S-mediated the regeneration of GSH pool is associated with the attenuation of MG toxicity by enhancing S-lactoglutathione and D-lactate production. Taken together, our results indicate complementary roles for H₂S and GSH to strengthen membrane integrity against Cr stress in maize seedlings.