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Identification of CDCA8, DSN1 and BIRC5 in Regulating Cell Cycle and Apoptosis in Osteosarcoma Using Bioinformatics

Qinwen Li1, Jie Liang1, Bo Chen1

  • 1Department of Orthopedics, 117899The People's Hospital of China Three Gorges University, The First People's Hospital of Yichang, Yichang City, Hubei, China.

Technology in Cancer Research & Treatment
|November 6, 2020
PubMed
Abstract

Insights

This study identified key genes like BIRC5, MAD2L1, and CDCA8 in osteosarcoma through bioinformatics analysis. These genes are potential targets for diagnosing and treating this bone cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Bioinformatics

Background:

  • Osteosarcoma is the most common bone tumor, but its molecular mechanisms remain unclear.
  • Identifying key genes and pathways is crucial for understanding osteosarcoma development.

Purpose of the Study:

  • To identify potential key genes and pathways in osteosarcoma using bioinformatics analysis.
  • To find molecular targets for osteosarcoma diagnosis and therapy.

Main Methods:

  • Downloaded and analyzed gene expression data (GSE14359) using bioinformatics tools.
  • Performed Gene Ontology and pathway enrichment analyses.
  • Constructed and analyzed a protein-protein interaction network.
  • Validated findings in osteosarcoma tissues and cell lines.

Main Results:

  • Identified 964 differentially expressed genes (DEGs) in osteosarcoma.
  • Highlighted 10 hub genes, including BIRC5, MAD2L1, and CDCA8, enriched in key signaling pathways.
  • Confirmed high expression of DSN1, BIRC5, CDCA8, MLF1IP, MAD2L1, and SPC24 in osteosarcoma tissues.
  • Demonstrated that CDCA8, DSN1, and BIRC5 promote osteosarcoma cell proliferation.

Conclusions:

  • Identified key genes (BIRC5, MAD2L1, CDCA8, DSN1, etc.) and pathways involved in osteosarcoma.
  • These findings suggest potential molecular targets and diagnostic biomarkers for osteosarcoma treatment.
Keywords:
bioinformatics analysisdifferentially expressed genesosteosarcoma

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