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Transcriptional profiling of embryo cryotolerance
Thamiris V Marsico1, Diana P Caetano2, Rafaela Rodrigues3
1Center for Natural and Human Sciences, Federal University of ABC, Santo André, São Paulo, Brazil.
Molecular Reproduction and Development
|November 6, 2020
Summary
High and low cryotolerant bovine embryos exhibit distinct molecular profiles. These differences in gene expression suggest unique strategies for overcoming cryopreservation stress and resuming development.
Area of Science:
- Reproductive Biology
- Genomics
- Embryology
Background:
- Cryosurvival of embryos is a complex process involving morphological, functional, and molecular changes.
- Understanding the molecular basis of embryo cryotolerance is crucial for improving assisted reproductive technologies.
Purpose of the Study:
- To evaluate the transcriptional profiling of bovine embryos with high (HC) and low (LC) cryotolerance.
- To identify molecular differences associated with the resumption of development after cryopreservation.
Main Methods:
- In vitro production and cryopreservation of bovine embryos (N=1137 produced, N=894 cryopreserved).
- RNA sequencing of blastocyst samples to assess transcriptional profiles.
- Differential gene expression analysis and pathway enrichment analysis.
Main Results:
- 114 genes were differentially expressed between HC and LC embryos.
- 27 genes were upregulated in HC embryos, while 84 genes were upregulated in LC embryos.
- HC embryos showed activated cellular growth, proliferation, and survival pathways, while LC embryos showed activated inflammatory processes and cell death pathways.
Conclusions:
- Distinct transcriptional profiles exist between HC and LC bovine embryos post-cryopreservation.
- Embryonic competence influences the transcriptome, with HC and LC embryos employing different molecular strategies to resume development after cryopreservation stress.
