Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Modification02:32

Histone Modification

15.3K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
15.3K
Histone Modification02:32

Histone Modification

4.1K
4.1K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

10.6K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
10.6K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

7.0K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.0K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.0K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.0K
Heterochromatin02:38

Heterochromatin

16.8K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
16.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Staged Buried Oral Mucosal Strip Urethroplasty for Long-Segment Post-Hypospadias Urethral Strictures: Achieving Functional Recovery and Distal Meatal Preservation.

International braz j urol : official journal of the Brazilian Society of Urology·2026
Same author

Multi-omics dissection of R-loop dynamics in tumorigenesis: From transcription-replication conflict to therapeutic targets.

Molecular therapy. Oncology·2026
Same author

Transcriptomic Signature and PROTAC Strategy Revealed Histone Lysine Demethylase as a Target of Anticancer Activity of Deferiprone.

ACS omega·2026
Same author

Empowering open medium-sized generative language models for effective structured search in biomedical systematic reviews.

International journal of medical informatics·2026
Same author

Population pharmacokinetic modeling and Monte Carlo simulation to optimize meropenem dosing in patients with severe postoperative infections.

Frontiers in pharmacology·2026
Same author

Harnessing Folate-Mediated PSMA Targeting for Precision Therapy: An Intelligent Liposomal Nanoplatform Against Prostate Cancer.

Pharmaceutics·2026

Related Experiment Video

Updated: Dec 1, 2025

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

7.8K

LSH mediates gene repression through macroH2A deposition.

Kai Ni1, Jianke Ren1, Xiaoping Xu1

  • 1Mouse Cancer Genetics Program, National Cancer Institute, Frederick, MD, 21702, USA.

Nature Communications
|November 7, 2020
PubMed
Summary

The LSH gene mutation causes ICF4 syndrome. LSH protein deposits histone variant macroH2A in chromatin to silence genes, a process disrupted in ICF4 patients.

More Related Videos

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.7K
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.4K

Related Experiment Videos

Last Updated: Dec 1, 2025

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

7.8K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.7K
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.4K

Area of Science:

  • Epigenetics and Chromatin Biology
  • Molecular Genetics
  • Human Disease Genetics

Background:

  • The LSH gene is implicated in the severe Immunodeficiency, Centromeric Instability, and Facial Anomalies (ICF) 4 syndrome.
  • LSH is a chromatin remodeling protein, but its in vivo function remains unclear.
  • Understanding LSH's role is crucial for ICF4 syndrome pathogenesis.

Purpose of the Study:

  • To elucidate the in vivo mechanism by which LSH modulates chromatin.
  • To investigate the relationship between LSH, histone variant macroH2A, and gene silencing.
  • To determine the molecular basis of ICF4 syndrome associated with LSH mutations.

Main Methods:

  • Chemical-induced proximity to recruit LSH to specific genomic loci.
  • Assessing macroH2A deposition via biochemical assays and ChIP-seq.
  • Analyzing transcriptional activity using reporter assays and gene expression analysis.
  • Utilizing siRNA to reduce macroH2A levels and evaluating phenotypic consequences.

Main Results:

  • Recruiting LSH to chromatin induces ATP-dependent deposition of macroH2A variants (macroH2A1.2 and macroH2A2).
  • LSH-mediated macroH2A deposition leads to transcriptional repression and gene silencing.
  • Loss of LSH or reduced macroH2A results in transcriptional reactivation at repeat sequences.
  • ICF4 patient cells exhibit impaired LSH function, reduced macroH2A deposition, and transcriptional reactivation.

Conclusions:

  • LSH is a key regulator of genome-wide macroH2A distribution and function.
  • LSH facilitates macroH2A insertion into chromatin, mediating transcriptional silencing.
  • Disrupted LSH-mediated macroH2A deposition is a pathogenic mechanism in ICF4 syndrome.