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Related Experiment Videos

Antigen expression during early human granulocyte development studied with immuno-electron microscopy.

R J Hogg1, A J Hodgson, D W Henderson

  • 1Adelaide Children's Hospital, South Australia.

Immunology and Cell Biology
|August 1, 1987
PubMed
Summary

Fucosyl-N-acetyllactosamine expression in human granulocyte precursors correlates with cellular development and rough endoplasmic reticulum growth. This antigen appears early in neutrophil differentiation but is not found in true plasma cells.

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Area of Science:

  • Hematology
  • Cell Biology
  • Immunology

Background:

  • Fucosyl-N-acetyllactosamine is a key carbohydrate antigen.
  • Understanding its expression during granulocyte development is crucial for hematological research.

Purpose of the Study:

  • To investigate the correlation between fucosyl-N-acetyllactosamine expression and ultrastructural development in human granulocyte precursors.
  • To identify the specific stages of granulocyte differentiation where this antigen is present.

Main Methods:

  • Utilized the monoclonal antibody FMC 10 for detection.
  • Employed immunogold techniques for ultrastructural analysis.
  • Examined human granulocyte precursors at various differentiation stages.

Main Results:

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  • Fucosyl-N-acetyllactosamine expression was detected from the myeloblast/early neutrophilic promyelocyte stage onwards.
  • Antigen presence correlated with significant development of the rough endoplasmic reticulum.
  • Low levels of the antigen were observed on monocytes, eosinophils, and some basophil precursors.
  • Promyelocyte differentiation showed rough endoplasmic reticulum changes resembling plasma cells, but these cells did not react with the antibody.

Conclusions:

  • Fucosyl-N-acetyllactosamine expression is a marker for early granulocyte differentiation and associated with endoplasmic reticulum development.
  • The antigen is specifically expressed in granulocyte precursors, distinguishing them from true plasma cells.