Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Natural occurrence and dietary risk assessment of Alternaria toxins in wheat flour and its products in Shanghai, China.

Food research international (Ottawa, Ont.)·2026
Same author

Optimization of Andrographolide Nanocrystal-loaded Liposomes by Box-Behnken Design and its <i>In vitro</i> and <i>In vivo</i> Evaluation.

Current drug delivery·2026
Same author

Approaches for enhancing bioavailability of macromolecular drugs.

International journal of pharmaceutics·2026
Same author

[<i>Qingshen</i> Granules alleviate renal fibrosis in mice with unilateral ureteral obstruction by regulating Akt3-mediated M1 macrophage polarization].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2026
Same author

Navigation System-Assisted vs Freehand Cannulated Screw Fixation for Femoral Neck Fractures: Protocol for a Multicenter Randomized Controlled Trial.

JMIR research protocols·2026
Same author

Regulating B-O Arrangement Boosts Anisotropy in a Simple Rubidium Borate, Leading to a High-Performance UV NLO Crystal.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Propylene carbonate-PVDF-HFP/MXene-based self-powered biosensor for auxiliary detection of salivary exosomal miRNA-155 in pediatric asthma.

Biosensors & bioelectronics·2026
Same journal

Nanostructured zinc-coordination supraparticles on cellulose fibers: A 3D-Printed μ-FAD integrated smartphone platform for multiplexed salivary metabolic monitoring.

Biosensors & bioelectronics·2026
Same journal

Reliable biomarker monitoring at microneedle aptamer biosensors using a dual-frequency ratiometric approach: Overcoming signal drifts.

Biosensors & bioelectronics·2026
Same journal

Interfacial structure-modified nanozyme drives single-receptor-single-reaction-unit multichannel sensor array for pesticide discrimination.

Biosensors & bioelectronics·2026
Same journal

A real-time 5-hydroxytryptamine monitoring system applicable both in vitro and in vivo.

Biosensors & bioelectronics·2026
Same journal

Recent developments of textile-based triboelectric nanogenerators for smart sports applications.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: Dec 1, 2025

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

47.1K

A CRISPR-driven colorimetric code platform for highly accurate telomerase activity assay.

Meng Cheng1, Erhu Xiong1, Tian Tian1

  • 1School of Life Science & College of Biophotonics, South China Normal University, Guangzhou, 510631, PR China.

Biosensors & Bioelectronics
|November 7, 2020
PubMed
Summary
This summary is machine-generated.

A novel CRISPR-Cas12a colorimetric system rapidly detects telomerase activity, overcoming limitations of the traditional Telomeric repeat amplification protocol (TRAP) assay. This method accurately identifies false-negatives in complex samples, improving clinical diagnostic potential.

Keywords:
AccuracyCRISPR-Cas12aCRISPR-Cas9Colorimetric codeLateral flow assayTelomerase activity

More Related Videos

Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

3.4K
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

10.3K

Related Experiment Videos

Last Updated: Dec 1, 2025

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

47.1K
Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

3.4K
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

10.3K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cancer Diagnostics

Background:

  • The Telomeric repeat amplification protocol (TRAP) is standard for telomerase activity assessment but is inhibited by complex biological matrices, causing false-negatives.
  • Existing TRAP methods require laborious electrophoresis, hindering rapid clinical application.
  • Internal controls improve TRAP reliability but add complexity and time.

Purpose of the Study:

  • To develop a rapid, user-friendly method for telomerase activity detection and false-negative identification.
  • To overcome the limitations of traditional TRAP assays in complex clinical samples.
  • To establish a colorimetric readout system for simplified telomerase activity analysis.

Main Methods:

  • A programmable CRISPR-Cas12a system combined with gold nanoparticles (AuNPs) was utilized for detecting telomeric repeat DNA and internal controls in TRAP products.
  • A colorimetric code system (Positive, Negative, False-Negative) was developed for naked-eye interpretation.
  • Cas9-mediated triple-line lateral flow assay (TL-LFA) was demonstrated for simultaneous detection on a single strip.

Main Results:

  • The developed platform enables rapid, naked-eye detection of telomerase activity and identification of false-negatives.
  • The system achieved high sensitivity (93.75%) and specificity (93.75%) in detecting telomerase activity in clinical liver cancer specimens.
  • Cas9-mediated TL-LFA demonstrated feasibility for convenient and accurate telomerase activity assay.

Conclusions:

  • The CRISPR-Cas12a colorimetric system offers a significant advancement for rapid and reliable telomerase activity detection.
  • This technology enhances diagnostic accuracy by identifying false-negatives in complex samples, crucial for clinical settings.
  • The developed platform, including TL-LFA, shows great promise for simplifying and improving telomerase activity assays in clinical diagnosis.