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Sperm shape abnormalities in mice exposed to californium-252 radiation
S N Goud1, J M Feola, Y Maruyama
1Department of Radiation Medicine, University of Kentucky Medical Center, Lexington 40536-0084.
Summary
Whole-body irradiation of male mice with 252Cf/60Co increased sperm abnormalities and decreased testis weight. The relative biological effectiveness (RBE) for 252Cf neutron radiation was 3.4, indicating significant biological impact.
Area of Science:
- Radiation biology
- Toxicology
- Spermatogenesis
Background:
- Male mice (B6C3F1 strain) were used to assess the biological effects of radiation.
- Sperm shape abnormalities are a sensitive indicator of reproductive toxicity.
Purpose of the Study:
- To evaluate the impact of mixed neutron and gamma radiation from 252Cf on sperm morphology and testis weight in male mice.
- To determine the relative biological effectiveness (RBE) of 252Cf radiation.
Main Methods:
- Whole-body irradiation of mice with varying doses of 252Cf/60Co.
- Spermatozoa were collected from the cauda epididymides 35 days post-irradiation.
- Sperm shape abnormalities were assessed using eosin-Y staining and light microscopy.
- Testis weight was measured.
Main Results:
- A dose-dependent increase in sperm shape abnormalities was observed in all irradiated groups compared to controls.
- The RBE for the mixed neutron and gamma radiation of 252Cf was determined to be 2.6.
- The RBE for the neutron component alone was calculated as 3.4.
- Irradiated animals showed a significant decrease in testis weight.
Conclusions:
- 252Cf/60Co radiation induces significant sperm shape abnormalities and testicular toxicity in male mice.
- The neutron component of 252Cf radiation exhibits a high relative biological effectiveness (RBE) for inducing these effects.