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Related Experiment Video

Updated: Dec 1, 2025

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Published on: July 24, 2018

Studying Seed Microbiomes.

Birgit Wassermann¹, Daria Rybakova¹, Eveline Adam¹

¹Institute of Environmental Biotechnology, Graz University of Technology, Graz, Austria.

Methods in Molecular Biology (Clifton, N.J.)

|November 8, 2020

Summary

This summary is machine-generated.

Understanding seed microbiomes is key to plant health. This study presents methods to analyze seed microbiota, aiding sustainable agriculture through new seed treatments and breeding strategies.

Keywords:

FISH-CLSM Germination Microbiota Peptide nucleic acid Real-time PCR Seed microbiome

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Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

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Last Updated: Dec 1, 2025

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Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

Published on: May 17, 2022

Area of Science:

Microbiology
Plant Science
Agricultural Science

Background:

Seed microbiomes significantly influence seed germination and overall plant performance.
The intricate relationship between seed microbiota and plant health remains incompletely understood.
Comprehensive analysis integrating various techniques is essential for a complete picture.

Purpose of the Study:

To provide a combined set of established and optimized methods for analyzing the seed microbiome.
To detail techniques for studying microbial communities associated with seeds.

Main Methods:

Culturing dormant seed microbiota.
Analyzing microbiota in germinated seeds, with and without substrate.
Quantifying microbial DNA using real-time PCR.
Host DNA depletion for amplicon and metagenome sequencing.
Visualizing seed endophytes via fluorescent in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM).

Main Results:

The chapter outlines a multi-faceted methodological approach for seed microbiome analysis.
The described methods cover cultivation, molecular quantification, and in-situ visualization.

Conclusions:

A thorough understanding of seed microbiome composition and function is crucial.
This knowledge can drive the development of novel seed treatments and breeding strategies.
These advancements support the goals of sustainable agriculture.