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Updated: Dec 1, 2025

Ecosystem Fabrication EcoFAB Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions
Published on: April 10, 2018
Protists in the Plant Microbiome: An Untapped Field of Research
Kenneth Dumack1, Michael Bonkowski2
1Cluster of Excellence on Plant Sciences (CEPLAS), Terrestrial Ecology Group, Institute of Zoology, University of Cologne, Köln, Germany. kenneth.dumack@uni-koeln.de.
Abstract:
Protists are mostly unicellular eukaryotes. Some protists are beneficial for plants, while others live as endosymbionts and can cause severe plant diseases. More detailed studies on plant-protist interactions exist only for plant pathogens and parasites. A number of protists live as inconspicuous endophytes and cause no visible disease symptoms, while others appear closely associated with the rhizosphere or phyllosphere of plants, but we still have only a vague understanding on their identities and functions. Here, we provide a protocol on how to assess the plant-associated protist community via Illumina-sequencing of ribosomal marker-amplicons and describe how to assign taxonomic affiliation to the obtained sequences.
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