An electrochemical aptasensor based on tetrahedral DNA nanostructures as a signal probe carrier platform for...

In this work, we proposed an electrochemical aptasensor for patulin (PAT) based on tetrahedral DNA nanostructures (TDNs) and thionine (Thi)-labeled Fe₃O₄ nanoparticles (Fe₃O₄NPs)/rGO signal amplification strategy. The rigid structure of TDNs could effectively improve the binding efficiency. Fe₃O₄NPs/rGO with excellent electrical conductivity and large specific surface area was used as a label material, which could load more Thi and accelerate electron transfer. Besides, the unique catalytic properties of Fe₃O₄NPs could achieve active signal amplification. Once PAT existed, PAT aptamer was released from the capture probe, thereby introducing Fe₃O₄NPs/rGO with Thi onto the electrode surface. Therefore, a noticeable increase in Thi current intensity was observed. Under the optimized conditions, the proposed aptasensor showed superior performance with a linear range from 5 × 10^-8 to 5 × 10^-1 μg mL^-1 and a detection limit of 30.4 fg mL^-1. The obtained sensor showed reliable specificity, stability and reproducibility, and was successfully applied to the determination of real samples.