IL-17C expression and its correlation with pediatric adenoids: a preliminary study

Area of Science:

• Immunology
• Otorhinolaryngology

Background:

• Interleukin-17C (IL-17C) is a cytokine involved in innate immunity and epithelial barrier function.
• IL-17C is upregulated early during infection and plays a role in immune responses.

Purpose of the Study:

• To investigate the expression profile of IL-17C in pediatric adenoids.
• To explore the role of IL-17C in the context of pediatric adenoid tissue and its association with bacterial exposure and allergic rhinitis.

Main Methods:

• Analysis of pediatric adenoid tissues and lavage fluids from 38 subjects.
• Evaluation of bacterial content using Limulus amebocyte lysate test and real-time PCR.
• Assessment of IL-17C and its receptor IL-17RE expression via western blot, immunohistochemistry, and real-time PCR.
• Measurement of Hsp27, Hsp70, and IL-17C levels in adenoid lavage fluid using ELISA.

Main Results:

• Pediatric adenoids harbor a normal bacterial flora and express the IL-17C receptor, IL-17RE.
• IL-17C was consistently expressed in adenoid tissues, regardless of allergic status.
• IL-17C secretion was detected in adenoid lavage fluid and correlated with elevated Hsp70 levels.

Conclusions:

• Pediatric adenoids express IL-17C, indicating a potential role in innate immunity modulation.
• The findings suggest IL-17C is an innate immunity-associated cytokine active in adenoid tissue.
• Adenoids may contribute to immune responses through the secretion of IL-17C.