Related Experiment Video

Updated: Dec 1, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.9K

MicroRNA-451a targets caveolin-1 in stomach cancer cells

Yi Wang1, Zhenmeng Lin1, Jintian Song1

  • 1Department of Gastrointestinal Surgery, Fujian Cancer Hospital & Fujian Medical University Cancer Hospital Fuzhou 350014, Fujian, China.

International Journal of Clinical and Experimental Pathology
|November 9, 2020
PubMed

Insights

MicroRNA-451 (miR-451) suppresses stomach cancer growth and metastasis by downregulating caveolin-1 (CAV1). Restoring CAV1 reverses these effects, indicating miR-451

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-451 (miR-451) is downregulated in stomach cancer, correlating with increased metastatic potential.
  • Caveolin-1 (CAV1) is implicated in cancer progression, and its regulation by miR-451 has been observed in other diseases.
  • The precise role of miR-451 in regulating stomach cancer cell functions via CAV1 remains to be elucidated.

Purpose of the Study:

  • To investigate whether miR-451 regulates stomach cancer cell biological functions through modulation of CAV1 expression.
  • To determine the therapeutic potential of miR-451 in stomach cancer treatment.

Main Methods:

  • Bioinformatic analysis to predict miR-451a-CAV1 interaction.
  • Dual-luciferase reporter assay to confirm the regulatory relationship.
  • Cell proliferation (MTT, EdU), cell cycle (PI), apoptosis (Annexin V-FITC/PI), migration, and invasion assays were performed on stomach cancer cells with altered miR-451a and CAV1 expression.

Main Results:

  • Bioinformatic analysis and luciferase assays confirmed that miR-451a negatively regulates CAV1 expression.
  • Overexpression of miR-451a in stomach cancer cells significantly inhibited cell proliferation, migration, and invasion, while promoting apoptosis.
  • Restoration of CAV1 expression reversed the inhibitory effects of miR-451a on these cellular processes.

Conclusions:

  • Overexpression of miR-451a restrains stomach cancer cell growth, migration, and invasion, and induces apoptosis.
  • miR-451a exerts its tumor-suppressive effects in stomach cancer by downregulating CAV1 expression.
  • miR-451a represents a potential therapeutic target for stomach cancer.
Keywords:
Caveolin-1MicroRNA-451astomach cancer

More Related Videos

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

7.8K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.4K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.4K
Drugs that Destabilize Microtubules01:10

Drugs that Destabilize Microtubules

Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...
2.4K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.3K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K