Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Riboswitches01:56

Riboswitches

9.2K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.2K
Experimental RNAi02:15

Experimental RNAi

6.9K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.9K
RNA Interference01:23

RNA Interference

27.3K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.3K
Types of RNA01:23

Types of RNA

71.3K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
71.3K
Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

837
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
837
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

358
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
358

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ribonucleic acid binding protein-mediated regulation of luteinizing hormone receptor expression in granulosa cells: relationship to sterol metabolism.

Molecular endocrinology (Baltimore, Md.)·2007
Same author

Psychological stress-induced oxidative stress as a model of sub-healthy condition and the effect of TCM.

Evidence-based complementary and alternative medicine : eCAM·2007
Same author

Overexpression of OsCOIN, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice.

Planta·2007
Same author

Edge-based scoring and searching method for identifying condition-responsive protein-protein interaction sub-network.

Bioinformatics (Oxford, England)·2007
Same author

[The value of long-term postoperative follow-up after curative resection of lung cancer and common problems associated with it].

Nihon Geka Gakkai zasshi·2007
Same author

Identification of a type III thioesterase reveals the function of an operon crucial for Mtb virulence.

Chemistry & biology·2007
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Dec 1, 2025

Author Spotlight: Investigating Wolbachia-Induced Thelytokous Parthenogenesis and Genetic Toolkit Development Through RNA Interference
05:29

Author Spotlight: Investigating Wolbachia-Induced Thelytokous Parthenogenesis and Genetic Toolkit Development Through RNA Interference

Published on: November 21, 2023

1.7K

The Copper-Controlled RNA Interference System in Trichoderma reesei.

Lei Wang1, Weixin Zhang1, Xiangfeng Meng2

  • 1State Key Laboratory of Microbial Technology, Shandong University, Qingdao, People's Republic of China.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel copper-controlled RNA interference (RNAi) system for Trichoderma reesei. This system allows reversible gene silencing, enabling better study of cellulase hyper-production mechanisms.

Keywords:
CopperKnockdownRNA interferenceReversibleT. reeseitcu1

More Related Videos

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum
11:00

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Published on: October 13, 2014

16.6K
Author Spotlight: Advancing Gene Silencing Research in Silkworms with dsRNA Delivery Through Feeding Chitosan Nanoparticles
05:36

Author Spotlight: Advancing Gene Silencing Research in Silkworms with dsRNA Delivery Through Feeding Chitosan Nanoparticles

Published on: October 4, 2024

2.4K

Related Experiment Videos

Last Updated: Dec 1, 2025

Author Spotlight: Investigating Wolbachia-Induced Thelytokous Parthenogenesis and Genetic Toolkit Development Through RNA Interference
05:29

Author Spotlight: Investigating Wolbachia-Induced Thelytokous Parthenogenesis and Genetic Toolkit Development Through RNA Interference

Published on: November 21, 2023

1.7K
Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum
11:00

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Published on: October 13, 2014

16.6K
Author Spotlight: Advancing Gene Silencing Research in Silkworms with dsRNA Delivery Through Feeding Chitosan Nanoparticles
05:36

Author Spotlight: Advancing Gene Silencing Research in Silkworms with dsRNA Delivery Through Feeding Chitosan Nanoparticles

Published on: October 4, 2024

2.4K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Fungal Genetics

Background:

  • Trichoderma reesei is a key industrial fungus for producing lignocellulose-degrading enzymes.
  • Understanding the molecular mechanisms of hyper-production, including transcriptional regulation and protein secretion, is crucial but limited by genetic tools.
  • Existing RNA interference (RNAi) systems in T. reesei lack control or rely on specific inducible promoters.

Purpose of the Study:

  • To develop a controllable and reversible RNA interference (RNAi) system in Trichoderma reesei.
  • To enable functional genomic studies for elucidating cellulase hyper-production mechanisms.
  • To demonstrate the utility of the system by targeting a key transcriptional activator.

Main Methods:

  • Development of a novel copper-controlled RNA interference (RNAi) system.
  • Implementation of reversible gene silencing in Trichoderma reesei.
  • Targeted knockdown of the xyr1 gene, a critical cellulase transcriptional activator.

Main Results:

  • A functional copper-controlled RNAi system was successfully established in T. reesei.
  • The system demonstrated reversible silencing of target genes.
  • The key transcriptional activator xyr1 was effectively knocked down, validating the system's proof of concept.

Conclusions:

  • The developed copper-controlled RNAi system provides a powerful and versatile tool for genetic manipulation in T. reesei.
  • This system facilitates deeper investigation into the molecular basis of cellulase hyper-production.
  • It opens new avenues for optimizing enzyme production in industrial fungi.