Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

665
Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
665

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription factor IRF-5 regulates lipid metabolism and mitochondrial function in murine CD8<sup>+</sup> T-cells during viral infection.

The EMBO journal·2025
Same author

Systemic administration of a viral nanoparticle neoadjuvant prevents lung metastasis development through emergency myelopoiesis.

Oncoimmunology·2024
Same author

Frizzled-6 promotes hematopoietic stem/progenitor cell mobilization and survival during LPS-induced emergency myelopoiesis.

Stem cell reports·2022
Same author

Cell-intrinsic Wnt4 ligand regulates mitochondrial oxidative phosphorylation in macrophages.

The Journal of biological chemistry·2022
Same author

Vangl2 Promotes Hematopoietic Stem Cell Expansion.

Frontiers in cell and developmental biology·2022
Same author

Persistent Cutaneous <i>Leishmania major</i> Infection Promotes Infection-Adapted Myelopoiesis.

Microorganisms·2022

Related Experiment Video

Updated: Dec 1, 2025

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
08:53

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Published on: August 31, 2016

15.7K

Experimental Competitive Bone Marrow Transplant Assays.

Roxann Hétu-Arbour1, Sarah Bouali1, Krista M Heinonen2

  • 1Institut National de la Recherche Scientifique, Laval, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2020
PubMed
Summary

This study details a competitive bone marrow transplant protocol in mice to assess hematopoietic stem cell (HSC) function. It outlines methods for evaluating HSC repopulation capacity and transplant efficiency for research into hematological malignancies.

Keywords:
Blood collectionBone marrow transplantationCompetitive Repopulating UnitFlow cytometryHematopoietic stem cellsMouse modelTotal body irradiation

More Related Videos

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model
10:11

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

Published on: March 7, 2013

15.7K
Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis
08:00

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

Published on: May 26, 2021

13.6K

Related Experiment Videos

Last Updated: Dec 1, 2025

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
08:53

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Published on: August 31, 2016

15.7K
Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model
10:11

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

Published on: March 7, 2013

15.7K
Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis
08:00

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

Published on: May 26, 2021

13.6K

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Immunology

Background:

  • Hematopoietic stem cells (HSCs) are crucial for producing all blood cell lineages.
  • Hematological malignancies can be treated by restoring normal hematopoiesis through bone marrow transplantation.
  • Mouse models are essential for studying HSC function and engraftment post-transplant.

Purpose of the Study:

  • To provide a detailed protocol for competitive bone marrow transplantation in mice.
  • To establish methods for evaluating long-term HSC function and repopulation capacity.
  • To offer insights into transplant efficiency calculations and experimental variations.

Main Methods:

  • Total body irradiation of recipient mice.
  • Preparation and administration of donor and competitor bone marrow cells.
  • Peripheral blood analysis for monitoring hematopoietic reconstitution.
  • Analysis of recipient bone marrow and secondary transplants for long-term HSC evaluation.

Main Results:

  • The protocol enables detailed assessment of HSC engraftment and competitive repopulation.
  • Established methods for calculating transplant efficiency are presented.
  • Variations and alternative procedures are discussed with their pros and cons.

Conclusions:

  • This protocol serves as a comprehensive guide for conducting competitive bone marrow transplant experiments in mice.
  • It facilitates the study of hematopoietic stem cell behavior and therapeutic potential in hematological disorders.
  • The detailed methodology supports reproducible research in stem cell transplantation and regenerative medicine.