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Published on: March 25, 2019
Liquid Phase Interfacial Surface-Enhanced Raman Scattering Platform for Ratiometric Detection of MicroRNA 155
Wei Luo1, Caijun Wu1, Siqi Huang1
1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Abstract:
The self-assembly of gold nanoparticles (Au NPs) on a liquid phase interface is often employed as a surface-enhanced Raman scattering (SERS) platform with advantages of simple preparation, high reproducibility, and a defect-free character, but they are limited to only detect a target with Raman signals. To overcome this problem, microRNA 155 without a Raman signal can be detected by a liquid phase interfacial ratiometric SERS platform. Compared with the typical solid phase SERS platform, we propose a distinctive strategy not only owning the advantages of the liquid phase interfacial platform but also breaking the limitation of recent liquid-liquid interfacial SERS analysis. This platform presents a fabulous sensitivity with a limit of detection (LOD) of 1.10 aM for microRNA 155. By simply altering the duplex-specific nuclease (DSN) enzyme amplification, our strategy can realize detection of a variety of microRNAs, paving the way to practical applications of a liquid phase SERS platform.
