Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rational Design of New Biocompatible Near-Infrared Conjugated Polymer Nanoparticles for Biomedical Applications.

Macromolecules·2026
Same author

Isolation and Biophysical Characterization of Lipoxygenase-1 from Soybean Seed, a Versatile Biocatalyst for Industrial Applications.

Biomolecules·2026
Same author

Polyphosphocholination of liposomic vehicles extends blood circulation, enhances cellular uptake, and lowers immunogenicity relative to PEGylation.

Journal of controlled release : official journal of the Controlled Release Society·2025
Same author

Novel ambipolar polymers for detection beyond 1000 nm with organic phototransistors.

Materials horizons·2025
Same author

Eco-Friendly Recovery of Homogalacturonan-Rich Pectin from Flaxseed Cake via NADES Extraction.

Polymers·2025
Same author

Co-Encapsulation of Phycocyanin and Albumin-Bound Curcumin in Biopolymeric Hydrogels.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Dec 1, 2025

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
15:33

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

29.5K

Structural Study of (Hydroxypropyl)Methyl Cellulose Microemulsion-Based Gels Used for Biocompatible Encapsulations.

Evdokia Vassiliadi1,2, Evgenia Mitsou1, Spyridon Avramiotis1

  • 1Institute of Chemical Biology, National Hellenic Research Foundation, 48, Vassileos Constantinou Ave., 11635 Athens, Greece.

Nanomaterials (Basel, Switzerland)
|November 10, 2020
PubMed
Summary

(Hydroxypropyl)methyl cellulose (HPMC) gels integrate water-in-oil microemulsions. These gels form channels within the HPMC matrix, hosting enzymes, but lack detectable microemulsion droplets after formation.

Keywords:
(hydroxypropyl)methyl cellulose (HPMC)electron paramagnetic resonance (EPR)lipasescanning electron microscopy (SEM)small angle X-ray scattering (SAXS)

More Related Videos

Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants
11:32

Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants

Published on: December 23, 2013

12.2K
Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
09:37

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Published on: October 26, 2009

37.4K

Related Experiment Videos

Last Updated: Dec 1, 2025

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
15:33

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

29.5K
Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants
11:32

Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants

Published on: December 23, 2013

12.2K
Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
09:37

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Published on: October 26, 2009

37.4K

Area of Science:

  • Materials Science
  • Colloid and Surface Chemistry
  • Biotechnology

Background:

  • (Hydroxypropyl)methyl cellulose (HPMC) forms gels incorporating water-in-oil (w/o) microemulsions.
  • These microemulsion-based gels (MBGs) are utilized as carriers for biocompatible ingredients.
  • The internal structure of these MBGs remains poorly understood.

Purpose of the Study:

  • To elucidate the structural organization of HPMC-based microemulsion gels.
  • To investigate the fate of microemulsion droplets upon incorporation into the HPMC matrix.
  • To determine the localization of enzymes within the gel structure.

Main Methods:

  • Scanning Electron Microscopy (SEM) for morphology.
  • Small-Angle X-ray Scattering (SAXS) for structural analysis and detection of reverse micelles.
  • Electron Paramagnetic Resonance (EPR) spectroscopy with spin probes for investigating polar/non-polar regions.
  • Enzyme-labelling technique for enzyme localization.

Main Results:

  • SEM revealed the bulk morphology of the microemulsion-based gels.
  • SAXS and EPR provided insights into the gel's structure and microenvironment.
  • No distinct microemulsion droplets were detected within the HPMC matrix post-incorporation.
  • A structural model proposes oil channels coated by surfactant and water layers, capable of hosting enzymes.

Conclusions:

  • The HPMC matrix undergoes structural rearrangement upon microemulsion incorporation.
  • While w/o microemulsions are crucial for gel formation, they are not present as discrete droplets in the final structure.
  • The HPMC gel forms a channeled network suitable for hosting active enzymes, suggesting potential applications in drug delivery and biomaterials.