Desalinization via freshwater restoration highly improved microbial diversity, co-occurrence patterns and functions...

Saltwater intrusion has greatly impacted the functions of coastal wetland soils worldwide by increasing the salt stress; desalinization via freshwater restoration has been suggested to recover saline wetland soils and biodiversity, but its effectiveness is debated. To evaluate the desalinization effectiveness, we characterized the microbial communities and activities using high throughput 16S rRNA gene sequencing and ¹⁵N isotopic techniques in freshwater restored (≥10 years) and unrestored wetlands, and then compared the data with reported values of original freshwater wetlands in one of the most dynamic coastal areas, Yellow river estuary (YRE). Our data revealed that freshwater input significantly increased the soil organic carbon (SOC; P < 0.05) after 10 years of restoration, yet it was still 10 times lower than the reported values of original freshwater wetlands. In general, microbial community showed higher diversities and more co-occurrence interactions in the restored than unrestored wetlands. The recovered phylogenetic diversity and the relative abundance of Chloroflexi (9.8-16.3%), Actinobacteria (5.5-10%), Latescibacteria (0.5-1.5%), Nitrospirae (0.9-1.4%) were up to the similar levels of original freshwater wetlands in YRE. Specifically, Gemmatimonadetes_denitrifier clones, as the representatives of denitrifiers, were recovered up to 0.3% with 20 times higher concomitant denitrification rate than anammox rate, significantly contributing to the nitrate removal in restored wetlands; however the rate will be reduced by 80% with a short-term saltwater intrusion. Our study highlighted that freshwater input effectively improved the microbial diversity and their functions and provided a good insight into the desalinization effectiveness via freshwater restoration in coastal wetlands worldwide. ORIGINALITY-SIGNIFICANCE STATEMENT: Salinization is globally spreading with approximately one billion hectares area covered with saline and/or sodic soils on the earth, and the negative effects of salinity on soil microbial communities and their activities have been frequently reported in previous studies all around the world; however, it remains largely unknown about whether the microbial communities and their activities can be recovered or not in soil suffered salinization. Desalinization via freshwater restoration is supposed to offer a good solution to soil salinization in coastal area, but the effectiveness is debated. Here, we are presenting the long-term of field study related to the desalinization effects on microbial diversity, co-occurrence and functions, and find desalinization via freshwater restoration can recover most of microbial communities up to the similar levels of that in original freshwater wetlands, and highly improved microbial diversity and their functions, which sheds a positive light on soil desalinization via freshwater restoration at microenvironments.