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Indicator displacement assays (IDAs): the past, present and future
Adam C Sedgwick1, James T Brewster, Tianhong Wu
1Department of Chemistry, The University of Texas at Austin, 105 East 24th Street A5300, Austin, Texas 78712-1224, USA. sessler@cm.utexas.edu anslyn@austin.utexas.edu.
Chemical Society Reviews
|November 10, 2020
Summary
Indicator displacement assays (IDAs) provide a versatile molecular sensing method, enabling detection of diverse analytes. This review explores IDA design strategies and their extensions, highlighting benefits and limitations for future research.
Area of Science:
- Analytical Chemistry
- Molecular Sensing
- Biotechnology
Background:
- Indicator displacement assays (IDAs) offer a unique molecular sensing approach.
- They detect diverse species, complex analytes, and mixtures, surpassing traditional sensors.
- Pioneering work by Inouye, Shinkai, and Anslyn spurred global development of IDA extensions.
Purpose of the Study:
- To review IDA design strategies and their recent extensions.
- To categorize IDAs based on target analytes and their significance.
- To discuss benefits and limitations of various IDA systems.
Main Methods:
- Review of existing literature on indicator displacement assays.
- Categorization of IDAs by target analyte and system design.
- Analysis of benefits and limitations for each IDA type.
Main Results:
- IDAs have evolved into various forms: eIDAs, FIDAs, RIAs, DDAs, IIDAs, AIDAs, MC-IDAs, and QDAs.
- IDA simplicity, low cost, high sensitivity, and automation compatibility drive their widespread use.
- The review details design strategies, benefits, and limitations across different IDA applications.
Conclusions:
- IDAs are a powerful and adaptable tool in molecular sensing.
- Further research into IDA design and application holds significant promise.
- Addressing current challenges can unlock new avenues for IDA development.
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