Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

1.8K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.8K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

14.2K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characteristics and management of asthma from the China Asthma Data Registry Project.

ERJ open research·2026
Same author

Macroscopic on-site evaluation based on core tissues length and weight during EBUS-TBNA using 22-gauge needles.

Chinese medical journal·2026
Same author

Integrated bulk and single-cell transcriptomics with experimental validation to identify calmodulin-related prognostic genes in lung adenocarcinoma.

American journal of cancer research·2026
Same author

ACOD1-itaconate in macrophage attenuates oxidative stress and inflammation in benign airway stenosis by upregulating and transferring FTH1.

Redox biology·2026
Same author

In Situ Synthesis of Biocompatible and Functionalizable Core-Shell Conductive Nanocomposites for Stretchable Electronics.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

The association between cardiovascular health, measured using Life's Essential 8, and obstructive sleep apnea: a cross-sectional and Mendelian randomization study.

American journal of translational research·2026

Related Experiment Video

Updated: Dec 1, 2025

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
08:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Published on: May 10, 2022

2.3K

Treg-dependent immunosuppression triggers effector T cell dysfunction via the STING/ILC2 axis.

Kalliopi Domvri1, Savvas Petanidis2, Paul Zarogoulidis3

  • 1Pulmonary Department-Oncology Unit, "G. Papanikolaou" General Hospital, Aristotle University of Thessaloniki, Thessaloniki 57010, Greece.

Clinical Immunology (Orlando, Fla.)
|November 11, 2020
PubMed
Summary

Oncogenic Kras signaling drives regulatory T cells (Tregs) to suppress the immune response in lung cancer, increasing metastasis. Targeting Tregs offers new strategies for inhibiting lung cancer spread, particularly in chemoresistant cases.

Keywords:
AICDILC2ImmunosuppressionKrasSTINGTreg

More Related Videos

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
07:36

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

7.2K
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

24.6K

Related Experiment Videos

Last Updated: Dec 1, 2025

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
08:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Published on: May 10, 2022

2.3K
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
07:36

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

7.2K
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

24.6K

Area of Science:

  • Immunology
  • Oncology
  • Cancer Research

Background:

  • Lung cancer is a leading cause of cancer death with low survival rates.
  • Aberrant Kras signaling in the lung tumor microenvironment drives regulatory T cells (Tregs), causing immune deregulation and immunosuppression.
  • The precise pathogenic mechanisms linking Kras, Tregs, and lung cancer progression are not fully understood.

Purpose of the Study:

  • To investigate the role of oncogenic Kras in Treg-mediated immunosuppression within the lung tumor microenvironment.
  • To explore the involvement of Tregs in tumor-associated metabolic reprogramming in lung cancer.
  • To elucidate the mechanisms by which Tregs contribute to lung cancer progression and metastasis.

Main Methods:

  • Investigated the impact of oncogenic Kras on Treg function in the lung tumor microenvironment.
  • Analyzed Treg-associated immunosuppressive pathways, including GATA3/NOS2 and STING inhibition.
  • Assessed the effects of Treg expression on T cell infiltration, effector cell function, and tumor metastasis in vivo.
  • Examined the correlation between Treg phenotypes (TIM3+/LAG3+) and chemoresistance, T cell dysfunction, and CD8 T cell exhaustion.

Main Results:

  • Oncogenic Kras signaling promotes Treg-mediated immunosuppression via GATA3/NOS2 and STING inhibition, reducing CD4+ T cell infiltration and increasing lung metastasis.
  • Enhanced Treg expression is linked to a low T/MDSC ratio and restricted CD8+ T effector cells, fostering a tumor-promoting environment.
  • TIM3+/LAG3+ Tregs contribute to Kras-driven chemoresistance and T cell dysfunction, correlating with CD8 T cell exhaustion and ILC2 augmentation.
  • Increased Treg activity promotes T lymphocyte activation-induced cell death (AICD) and facilitates lymph node metastasis.

Conclusions:

  • Tregs play multifaceted roles in sustaining lung immunosuppressive neoplasia by remodeling the tumor microenvironment.
  • Findings reveal novel Treg-dependent mechanisms driving lung cancer progression, immune evasion, and metastasis.
  • Targeting Treg-mediated immunosuppression presents a promising therapeutic strategy for inhibiting metastasis, especially in chemoresistant lung tumors.