Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle

Area of Science:

• Biochemistry
• Plant Science
• Analytical Chemistry

Background:

• Trans-zeatin is a crucial active phytohormone in immature corn kernels.
• Accurate detection of trans-zeatin is vital for understanding plant growth and development.

Purpose of the Study:

• To develop a highly sensitive and selective aptamer-based colorimetric method for trans-zeatin detection.
• To establish a simple and visually observable assay for quantifying trans-zeatin.

Main Methods:

• Construction of an aptamer sequence that binds specifically to trans-zeatin.
• Utilizing gold nanoparticles (AuNPs) protected by complementary DNA, which undergo a color change (red to blue) upon binding.
• Employing a duplex-to-aptamer structure switching mechanism triggered by trans-zeatin presence.

Main Results:

• The method demonstrated high sensitivity with a linear detection range from 0.05 to 0.75 μM and a detection limit of 0.037 μM.
• The color change of AuNPs was observable by the naked eye, indicating the presence and concentration of trans-zeatin.
• The assay successfully detected trans-zeatin in real plant samples, showcasing its practical applicability.

Conclusions:

• A novel, simple, and sensitive aptamer-based colorimetric method for trans-zeatin detection has been successfully developed.
• This method offers a reliable tool for quantifying trans-zeatin in plant tissues.
• The visual detection capability and successful application in real samples highlight the potential of this assay in plant science research.