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Mitotically associated long non-coding RNA is a tumor promoter in anaplastic thyroid cancer

Nai-Si Huang1,2, Bo-Wen Lei1,2, Li-Cheng Tan1,2

  • 1Department of Head and Neck Surgery, Fudan University, Shanghai Cancer Center, Shanghai, China.

Annals of Translational Medicine
|November 12, 2020
PubMed
Abstract

Insights

Mitotically associated long non-coding RNA (MANCR) promotes anaplastic thyroid cancer (ATC) growth and invasion. MANCR may be a potential therapeutic target for treating this deadly cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Anaplastic thyroid cancer (ATC) is a deadly malignancy with poor therapeutic response.
  • The role of long non-coding RNAs (lncRNAs) in ATC development is not well understood.
  • Identifying novel molecular targets is crucial for effective ATC treatment.

Purpose of the Study:

  • To investigate the lncRNA expression profile in ATC.
  • To identify potential lncRNAs as therapeutic targets for ATC.
  • To elucidate the function of dysregulated lncRNAs in ATC carcinogenesis.

Main Methods:

  • Whole transcriptome sequencing of ATC and normal thyroid samples.
  • Analysis of public datasets (GEO, TCGA) for clinical validation.
  • In vitro assays (proliferation, Transwell, apoptosis) in ATC cell lines.
  • Gene Ontology (GO) and gene set enrichment analysis (GSEA) for pathway identification.

Main Results:

  • 182 differentially expressed lncRNAs identified in ATC.
  • Mitotically associated long non-coding RNA (MANCR) was significantly overexpressed in ATC.
  • MANCR depletion inhibited ATC cell proliferation and invasion, and induced apoptosis.
  • MANCR co-expressed genes were primarily involved in mitosis and cell cycle regulation.

Conclusions:

  • MANCR acts as a tumor promoter in ATC, potentially through cell cycle regulation.
  • MANCR's minimal expression in normal tissues suggests its potential as a targeted therapy for ATC.
  • Further research into MANCR's role could lead to novel treatment strategies for anaplastic thyroid cancer.
Keywords:
Long non-coding RNA (lncRNA)anaplasticoncogenesisthyroid carcinoma

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