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Updated: Nov 30, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
Published on: December 11, 2020
CRISPR-ERA: A Webserver for Guide RNA Design of Gene Editing and Regulation
Honglei Liu1,2, Xiaowo Wang3,4,5,6
1School of Biomedical Engineering, Capital Medical University, Beijing, China. liuhonglei@ccmu.edu.cn.
Abstract:
The CRISPR/Cas9 system has been developed as a powerful technology for both targeted genome editing and gene regulation. However, the design of efficient single-guide RNAs (sgRNAs) remains challenging with the consideration of many criteria. In this section, we introduce how to design sgRNA sequences and build genome-wide sgRNA library using CRISPR-ERA, which is one of the state-of-the-art designer webserver tools for sgRNA design based on a set of sgRNA design rules summarized from published reports.
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