Related Experiment Video
Updated: Nov 30, 2025
An Open-Source Normothermic Perfusion System Designed for Research Scientists
Published on: July 18, 2025
Establishment and optimization of a high-throughput mimic perfusion model in ambr® 15
Lu Jin1, Zhen-Shou Wang2, Yun Cao2
1School of Life Science and Technology, China Pharmaceutical University, #639 Longmian Dadao, Jiangning District, Nanjing, 211198, Jiangsu, People's Republic of China.
Objectives:
To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system.
Results:
An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM couldn't reach the viable cell density (VCD) at a bench scale bioreactor (BR), it maintained VCD at approximately 30 × 106 cells/mL with a cell bleeding rate estimated theoretically and predicted the cell specific perfusion rate (CSPR). A base-feeding strategy was developed to alleviate the pH drop during sedimentation which would adversely have an impact on cell growth, and showed an apparent cell viability improvement from 79.6% (control) to 90.1% on Day 18. The optimized SDM for mimic perfusion was employed for media screening in two cell lines.
Conclusions:
A small-scale high-throughput perfusion model in ambr® 15 was developed, optimized to improve cell viability, and as a result, utilized for media screening in two cell lines.
