Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

947
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
947
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

1.1K
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
1.1K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.3K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.3K
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

1.3K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
1.3K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

518
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
518
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

459
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
459

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Probing the Enhancement of <math><semantics><mrow><msup><mrow></mrow> <mrow><mn>1</mn></mrow></msup></mrow> <annotation>$$ {}^1 $$</annotation></semantics></math> H NMR Relaxation Rate in Hydrated Boehmite Systems.

Magnetic resonance in chemistry : MRC·2026
Same author

Molybdocene dichloride intercalation into zirconium phosphate nanoparticles.

Journal of organometallic chemistry·2026
Same author

Data-driven classification of tissue water populations by massively multidimensional diffusion-relaxation correlation MRI.

Frontiers in neuroscience·2026
Same author

Effect of Mg<sup>2+</sup> Ions on Transport Properties in Molten Chloride Fast Reactor Fuels.

The journal of physical chemistry. B·2026
Same author

Complementarity of BOLD and ADC-fMRI in Mapping Brain Visual Processing in the Rat.

NMR in biomedicine·2026
Same author

Frequency-Dependent Diffusion-Relaxation Distribution MRI: Scan-Rescan Reproducibility Ex Vivo and Caveats.

NMR in biomedicine·2025

Related Experiment Video

Updated: Nov 30, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.8K

Iterative baseline correction algorithm for dead time truncated one-dimensional solid-state MAS NMR spectra.

Maxime Yon1, Franck Fayon1, Dominique Massiot1

  • 1CNRS, CEMHTI UPR3079, Université d'Orléans, F-45071, Orléans, France.

Solid State Nuclear Magnetic Resonance
|November 15, 2020
PubMed
Summary

This study introduces an automated algorithm to correct rolling baselines in solid-state Nuclear Magnetic Resonance (NMR) spectra. The method accurately corrects spectral distortions without altering data, improving quantification.

Keywords:
Baseline correctionMASQuantificationSS-NMR

More Related Videos

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.4K
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.4K

Related Experiment Videos

Last Updated: Nov 30, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.8K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.4K
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.4K

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Data Processing and Analysis

Background:

  • Pulse-acquire one-dimensional Magic Angle Spinning (MAS) NMR spectra often suffer from rolling baseline artifacts.
  • These artifacts are primarily induced by time-domain truncation due to instrumental dead time.
  • Accurate baseline correction is crucial for reliable spectral deconvolution and quantification.

Purpose of the Study:

  • To develop and present an automated algorithm for correcting rolling baseline distortions in MAS NMR spectra.
  • To provide a method that is independent of NMR resonance line shapes and widths.
  • To offer a tool that preserves the integrity of the acquired free induction decay (FID) data.

Main Methods:

  • An iterative baseline estimation approach is employed, constrained by the dead time duration in the time domain.
  • A histogram filter is utilized for adaptive selection of baseline points.
  • The algorithm operates directly on the time-domain data without modifying FID points.

Main Results:

  • The algorithm successfully corrects rolling baselines in synthetic solid-state spectra of Fluorine-19 (19F), Gallium-71 (71Ga), and Sodium-23 (23Na).
  • Accuracy was validated by comparing fitted baselines against theoretical baselines.
  • The method demonstrated versatility on additional 23Na and 71Ga solid-state spectra.

Conclusions:

  • The developed algorithm provides an effective and accurate solution for rolling baseline correction in MAS NMR.
  • Its independence from spectral line shape assumptions and non-modification of FID data ensure suitability for precise spectral quantification.
  • The algorithm is accessible via a user-friendly standalone Matlab® application.