Related Experiment Video
Updated: Nov 30, 2025
07:13
ATAC-Seq Optimization for Cancer Epigenetics Research
Published on: June 30, 2022
5.0K
Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation
Steven Henikoff1,2, Jorja G Henikoff1, Hatice S Kaya-Okur1
1Basic Sciences Division Fred Hutchinson Cancer Research Center, Seattle, United States.
Elife
|November 16, 2020
Summary
Chromatin accessibility mapping can now be performed using modified CUT&Tag protocols. This method generates high-quality accessibility maps alongside other epigenetic marks, identifying transcription-coupled regulatory sites.
Area of Science:
- Epigenetics
- Molecular Biology
- Genomics
Background:
- Chromatin accessibility mapping reveals regulatory elements in the genome.
- ATAC-seq and CUT&Tag are established tagmentation-based epigenomic profiling methods.
- CUT&Tag uses antibody-tethered transposase for targeted profiling.
Purpose of the Study:
- To develop a method for generating chromatin accessibility maps using CUT&Tag.
- To enable parallel profiling of chromatin accessibility and other epigenetic marks.
- To identify transcription-coupled accessible regulatory sites.
Main Methods:
- Modification of tagmentation conditions for H3K4me2/H3K4me3 CUT&Tag.
- Antibody-tethered tagmentation of accessible DNA sites.
- Integration of all steps from nuclei to library preparation in single PCR tubes.
Main Results:
- Modified CUT&Tag produces chromatin accessibility maps comparable to ATAC-seq.
- Parallel profiling of accessibility and other epitopes is feasible.
- The method successfully identifies transcription-coupled accessible regulatory sites.
Conclusions:
- Modified CUT&Tag offers a versatile approach for epigenomic profiling.
- This method simplifies library preparation and enables simultaneous analysis of multiple epigenetic features.
- It provides insights into transcription-coupled regulatory element accessibility.
