Increasing the penetration depth of temporal focusing multiphoton microscopy for neurobiological applications
Christopher J Rowlands1,2, Oliver T Bruns3, Daniel Franke4
1Department of Bioengineering, Imperial College London, London SW7 2AZ, United Kingdom.
Abstract:
The first ever demonstration of temporal focusing with short wave infrared (SWIR) excitation and emission is demonstrated, achieving a penetration depth of 500 µm in brain tissue. This is substantially deeper than the highest previously-reported values for temporal focusing imaging in brain tissue, and demonstrates the value of these optimized wavelengths for neurobiological applications.


