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Whole Mount In Situ Hybridization in Murine Tissues

Deepika Sharma1, Matthew J Hilton2, Courtney M Karner3,4

  • 1Department of Orthopaedic Surgery, Duke Orthopaedic, Cellular, Developmental and Genome Laboratories, Duke University School of Medicine, Durham, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 16, 2020
PubMed

Keywords:
DigoxigeninGene expressionIn situ hybridizationRNAWISH

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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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