Related Experiment Video
Updated: Nov 30, 2025

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
Published on: March 1, 2012
Cauliflower mosaic virus P6 inclusion body formation: A dynamic and intricate process
Roberto Alers-Velazquez1, Sarah Jacques1, Clare Muller1
1Department of Biological Sciences, University of Toledo, 2801 West Bancroft Street, Mail Stop 601, Toledo, OH, 43606, USA.
Abstract:
During an infection, Cauliflower mosaic virus (CaMV) forms inclusion bodies (IBs) mainly composed of viral protein P6, where viral activities occur. Because viral processes occur in IBs, understanding the mechanisms by which they are formed is crucial. FL-P6 expressed in N. benthamiana leaves formed IBs of a variety of shapes and sizes. Small IBs were dynamic, undergoing fusion/dissociation events. Co-expression of actin-binding polypeptides with FL-P6 altered IB size distribution and inhibited movement. This suggests that IB movement is required for fusion and growth. A P6 deletion mutant was discovered that formed a single large IB per cell, which suggests it exhibited altered fusion/dissociation dynamics. Myosin-inhibiting drugs did not affect small IB movement, while those inhibiting actin polymerization did. Large IBs colocalized with components of the aggresome pathway, while small ones generally did not. This suggests a possible involvement of the aggresome pathway in large IB formation.
More Related Videos
08:14Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
Published on: February 14, 2013
08:56Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Related Concept Videos
Protein Complex Assembly
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Viral Structure
Subviral Agents
Intralumenal Vesicles and Multivesicular Bodies
Intracellular Movement of Viruses and Bacteria
Viral Recombination