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Upconversion nanoparticles coated with molecularly imprinted polymers for specific sensing.

Ling Yang1, Xiaorui Chen, Ping'an Ma

  • 1State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China. jlin@ciac.ac.cn zycheng@ciac.ac.cn.

Dalton Transactions (Cambridge, England : 2003)
|November 17, 2020
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Summary
This summary is machine-generated.

We developed a novel fluorescent sensor using lanthanide-doped upconversion nanoparticles (UCNPs) and molecular imprinting for accurate biomarker detection. This method enhances specificity and sensitivity for detecting target molecules like rhodamine B.

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Area of Science:

  • Nanotechnology
  • Biomarker Detection
  • Luminescent Sensors

Background:

  • Lanthanide-doped luminescent nanoparticles, particularly upconversion nanoparticles (UCNPs), show promise for biomarker detection due to their photostability and low background fluorescence.
  • Current UCNP-based sensors using Fluorescence Resonance Energy Transfer (FRET) suffer from low accuracy due to non-specific adsorption of target molecules.
  • Accurate sensing of specific molecules is crucial for various diagnostic and analytical applications.

Purpose of the Study:

  • To develop a highly specific and accurate fluorescent sensing platform for target molecules.
  • To overcome the limitations of superficial adsorption in traditional UCNP-based FRET assays.
  • To integrate molecular imprinting with upconversion luminescence for enhanced molecular detection.

Main Methods:

  • Synthesized UCNPs functionalized with a molecularly imprinted polymer (MIP) layer.
  • Utilized the MIP layer for selective capture of the target molecule, rhodamine B.
  • Measured changes in upconversion luminescence signal due to FRET between UCNPs and captured rhodamine B.

Main Results:

  • The synthesized nanostructure demonstrated specific capture of rhodamine B via imprinted cavities.
  • A concentration-dependent change in upconversion luminescence signal was observed.
  • Quantitative analysis confirmed adherence to the Stern-Volmer equation with a limit of detection of 6.27 μg mL⁻¹.

Conclusions:

  • The developed fluorescence sensing approach effectively combines upconversion luminescence and molecular imprinting technologies.
  • This integrated method offers high specificity and accuracy for detecting target molecules.
  • The sensor shows significant potential for sensitive and selective molecular detection in various applications.