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Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

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Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
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Related Experiment Video

Updated: Nov 30, 2025

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood
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A Heparin based dual ratiometric sensor for Thrombin.

Niyati H Mudliar1, Prabhakar M Dongre1, Prabhat K Singh2

  • 1Department of Biophysics, University of Mumbai, Vidyanagari, Kalina, Mumbai 400098, India.

International Journal of Biological Macromolecules
|November 17, 2020
PubMed
Summary

This study introduces a simple, label-free fluorescence sensor for detecting thrombin (a key clotting enzyme). The novel method uses Thioflavin-T and heparin, offering a cost-effective and sensitive approach for disease diagnostics.

Keywords:
Molecular rotorsRatiometric fluorescence sensorThrombin sensor

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Thrombin is a critical enzyme in blood coagulation, and its dysregulation is linked to various diseases like thrombosis and haemophilia.
  • Current fluorescence-based thrombin detection methods often require expensive, sophisticated techniques involving labeled assays or specific biomolecules (aptamers, antibodies).

Purpose of the Study:

  • To develop a simple, selective, sensitive, and label-free fluorescence detection scheme for thrombin.
  • To provide a cost-effective alternative to existing thrombin detection assays.

Main Methods:

  • Utilized a fluorescent complex of Heparin with the molecular rotor dye Thioflavin-T.
  • Exploited the specific electrostatic interaction between cationic thrombin and anionic heparin to modulate the Thioflavin-T-Heparin system's equilibrium.
  • Employed a ratiometric fluorescence response for quantification.

Main Results:

  • Demonstrated a simple, selective, and sensitive label-free fluorescence detection of thrombin.
  • The developed scheme offers robust quantification even in complex biological media, including diluted serum.
  • All components used are commercially available, enhancing practicality.

Conclusions:

  • The proposed Thioflavin-T-Heparin based fluorescence detection scheme provides a practical and cost-effective method for thrombin sensing.
  • This label-free approach has potential applications in clinical diagnostics for thrombin-related disorders.