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Updated: Nov 30, 2025

Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
Published on: June 2, 2017
In Vitro Destruction of Pathogenic Bacterial Biofilms by Bactericidal Metallic Nanoparticles via Laser-Induced
Alena Nastulyavichus1, Eteri Tolordava1,2, Andrey Rudenko1
1Laboratory of Laser Nanophysics and Biomedicine, P.N. Lebedev Physical Institute of the Russian Academy of Sciences, 119991 Moscow, Russia.
Abstract:
A novel, successful method of bactericidal treatment of pathogenic bacterial biofilms in vitro by laser-induced forward transfer of metallic nanoparticles from a polyethylene terephthalate polymeric substrate was suggested. Transferred nanoparticles were characterized by scanning and transmission electron microscopy, energy-dispersive X-ray and Raman spectroscopy. The antibacterial modality of the method was tested on Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas Aeruginosa) bacterial biofilms in vitro, revealing their complete destruction. The proposed simple, cost-effective and potentially mobile biofilm treatment method demonstrated its high and broad bactericidal efficiency.
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