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Fundamental Study of Decellularization Method Using Cyclic Application of High Hydrostatic Pressure.
Daiki Zemmyo1, Masashi Yamamoto1, Shogo Miyata2
1Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.
Micromachines
|November 18, 2020
Summary
Cyclic high hydrostatic pressure (HHP) offers a novel method for decellularization. This technique effectively removes cellular material and DNA without damaging the extracellular matrix, preserving tissue structure for regenerative medicine applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Decellularized tissues, composed of extracellular matrices (ECMs), are crucial for regenerative medicine.
- High hydrostatic pressure (HHP) is a physical method for decellularization, but ultrahigh pressures can denature ECM structure.
Purpose of the Study:
- To investigate the effects of cyclic HHP on cell membrane structure.
- To develop a novel decellularization method that avoids ECM denaturation.
- To establish a decellularization technique preserving ECM integrity.
Main Methods:
- Development of a decellularization device utilizing cyclic HHP (max 250 MPa, 5 cycles).
- Application of cyclic HHP to NB1RGB cell suspension.
- Evaluation of intracellular DNA content and cell morphology post-treatment.
Main Results:
- Cyclic HHP significantly reduced intracellular DNA compared to static HHP.
- Cyclic HHP promoted the disruption of cell and nuclear membranes.
- Cellular structures were denatured and destroyed at lower pressure levels than previously reported.
Conclusions:
- Cyclic HHP is a viable method for decellularization.
- This technique offers a way to decellularize tissues without ECM denaturation.
- The findings support the development of improved biomaterials for tissue regeneration.
