Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.2K
Next-generation Sequencing03:00

Next-generation Sequencing

96.3K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
96.3K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

20.0K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
20.0K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

6.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.7K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.0K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.0K
Sanger Sequencing01:57

Sanger Sequencing

768.6K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
768.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Vertebrate Genomes Project Phase I: A global reference genome resource.

bioRxiv : the preprint server for biology·2026
Same author

Factors Influencing Consultant Knee Surgeons' Decision Making in Anterior Cruciate Ligament (ACL) Injury Management in Athletes: An International Delphi Study.

Sports medicine (Auckland, N.Z.)·2026
Same author

Ancient DNA from shells reveals delayed genomic erosion and rapid immune adaptation in the critically endangered black abalone.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Genome-wide discovery and characterization of terpene synthases contributing to strawberry aroma metabolism.

Plant physiology·2026
Same author

Fc gamma receptor binding modulates IgG clearance in cancer cachexia.

Frontiers in immunology·2026
Same author

Adoption of Standard Reference SNP Identifiers in Agricultural Genomics for Interoperability and Data Reuse.

Scientific data·2026

Related Experiment Video

Updated: Nov 29, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

23.5K

Highly accurate long-read HiFi sequencing data for five complex genomes.

Ting Hon1, Kristin Mars1, Greg Young1

  • 1Pacific Biosciences of California Inc., 1305 O'Brien Dr., Menlo Park, CA, 94025, USA.

Scientific Data
|November 18, 2020
PubMed
Summary
This summary is machine-generated.

High-accuracy, long-read sequencing data from PacBio HiFi technology is now available. These datasets aid in developing new bioinformatics tools for complex genome analysis and variant detection.

More Related Videos

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.6K
Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

8.9K

Related Experiment Videos

Last Updated: Nov 29, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

23.5K
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.6K
Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

8.9K

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • PacBio HiFi sequencing provides highly accurate long reads (>99.5% accuracy, 10-25 kb read length).
  • Accurate long reads are crucial for advancing complex genomic applications.
  • A need exists for benchmark datasets to develop and evaluate bioinformatics tools.

Purpose of the Study:

  • To present deep coverage HiFi datasets for diverse and complex genomes.
  • To provide resources for the development of genome assemblers, variant callers, and haplotyping algorithms.
  • To facilitate the evaluation of PacBio HiFi sequencing for challenging genomic studies.

Main Methods:

  • Generation of high-fidelity (HiFi) long-read sequencing data using the PacBio Sequel II System.
  • Sequencing of five complex samples: Mus musculus, Zea mays, Fragaria × ananassa (octoploid), Rana muscosa (diploid), and a mock metagenome community.
  • Deep coverage sequencing to ensure comprehensive data for algorithm development.

Main Results:

  • Release of comprehensive HiFi datasets for model and complex organisms.
  • Inclusion of data for polyploid and highly repetitive genomes.
  • Provision of a mock metagenome dataset for microbiome research.

Conclusions:

  • The presented datasets are valuable resources for the genomics and bioinformatics communities.
  • These data enable unrestricted development and testing of novel bioinformatic tools.
  • The datasets support research into complex genome structure, evolution, and variant detection.