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Updated: Nov 29, 2025
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Do I Have HIV or Not? Lack of RNA Detection and the Case for Sensitive DNA Testing
Sandra A Springer1,2, Silvina Masciotra3, Jeffrey A Johnson3
1Section of Infectious Disease, Department of Internal Medicine, Veterans Administration Connecticut Healthcare System, West Haven, Connecticut, USA.
Abstract:
We present a case of a 20-year-old male who had ambiguous HIV test results after entering new provider care and whose status was later complicated by undetectable viral RNA off antiretroviral therapy (ART). Verifying HIV infection status may occasionally require sensitive DNA testing that might need to be considered in diagnostic guidelines to resolve diagnosis and ensure appropriate ART management.
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