Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Retraction Note: NSD2 targeting reverses plasticity and drug resistance in prostate cancer.

Nature·2026

Same author

Smoking prevalence in Italy from 1993 to 2024: a trend analysis framed by public health policies and emerging consumption behaviors.

Journal of public health (Oxford, England)·2026

Same author

Identifying Two New Ros/MucR Proteins: An Atypical Structure with a Divergent Function.

Biomolecules·2026

Same author

Effects of polygenic risk score communication on short term health outcomes: systematic review and meta-analysis.

BMJ medicine·2026

Same author

Molecular Tumor Boards clinical impact on patient care and structural features: A systematic review and meta-analysis.

PLoS medicine·2026

Same author

Engineering a PD-L1-sensing synthetic receptor for programmable macrophage response.

Journal of biological engineering·2026

Same journal

Patient-derived organoids reveal ductal dysfunction and CFTR-modulator responses in chronic pancreatitis.

Cell stem cell·2026

Same journal

Lineage plasticity driven by GATA6 loss fuels colorectal cancer metastasis.

Cell stem cell·2026

Same journal

Quantitative molecular cartography of emergency myelopoiesis reveals conserved modules of hematopoietic activation.

Cell stem cell·2026

Same journal

Paired pre- and post-transplant human immunoprofiling identifies an IFN-γ-JAK1 axis limiting stem-cell-derived RPE engraftment.

Cell stem cell·2026

Same journal

ENPP1 blockade with a humanized monoclonal antibody enhances renal repair after acute kidney injury.

Cell stem cell·2026

Same journal

ZNF512B safeguards genome integrity at regulatory regions to repress the SASP and inflammation.

Cell stem cell·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 29, 2025

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Published on: September 28, 2019

Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation.

Martin Bergert¹, Sergio Lembo¹, Sumana Sharma²

¹Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.

|November 18, 2020

Summary

This summary is machine-generated.

Cell surface mechanics regulate stem cell differentiation. Decreased membrane-to-cortex attachment allows mouse embryonic stem cells to exit naive pluripotency, a process reversible by mechanical tethering.

Keywords:

atomic force spectroscopy exit from pluripotency mESC membrane tension membrane-to-cortex attachment (MCA)naive-to-primed transition

More Related Videos

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

Published on: October 1, 2007

Related Experiment Videos

Last Updated: Nov 29, 2025

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Published on: September 28, 2019

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

Published on: October 1, 2007

Area of Science:

Cell Biology
Biophysics
Developmental Biology

Background:

Cell differentiation involves shape changes requiring altered surface mechanics.
The role of cell surface mechanics in controlling differentiation remains largely unknown.

Purpose of the Study:

To investigate whether cell surface mechanics, specifically membrane-cortex attachment, regulate the exit from naive pluripotency in mouse embryonic stem cells.

Main Methods:

Measurement of membrane tension during early stem cell differentiation.
Mechanical manipulation of plasma membrane-to-cortex attachment via Ezrin activity modulation and synthetic linkers.

Main Results:

Naive stem cells decrease plasma membrane-to-cortex attachment during the transition to a primed state.
Preventing this detachment by mechanical tethering forces stem cells to maintain naive pluripotency.

Conclusions:

Reduced membrane-to-cortex attachment is a novel cell-intrinsic mechanism essential for exiting pluripotency.
Membrane mechanics act as a critical gatekeeper for stem cell differentiation.