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First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China
Yang Yang1,2, Yifan Sun2, Qingliang Li2
1College of Agriculture, Nanjing Agriculture University, Nanjing, 210095, People's Republic of China.
Background:
Virus disease is one of the main diseases in grapevine, and there has been no report on Plum bark necrosis and stem pitting-associated virus infecting grapevine in China.
Objective:
The leaf samples of grapevine cultivar 'Cabernet Gernischt' were collected from Shandong province, which the leaves suffered from viral-like symptoms with spotting and crinkle.
Methods:
Small RNA-seq combined with reverse transcription PCR (RT-PCR) were performed to detect the potential viruses in these field samples. Phylogenetic tree was constructed using the neighbor joining method in MEGA 5.1 CONCLUSIONS: This is the first report of PBNSPaV infecting grapevine in China, contributing to a better understanding of the epidemiology and host range distribution of this pathogen.
