[Studies on using choline salt of N-glucosylpolyfungine as an antifungal preparation for cell culture]

Fungistatic and fungicidal activity as well as the toxicity of the polyfungine derivative of choline N-glucosylpolyfungine salt for chicken embryo fibroblasts were studied. This preparation was found to inhibit the growth of Candida albicans, Aspergillus niger and Penicillium sp. at 25-100 micrograms/ml concentrations of the medium. The highest sensitivity to polyfungine was shown by C. albicans strain, and the lowest by Aspergillus niger strain. The preparation studied did not lose its activity in the medium containing 20% of calf serum. Its toxicity for cells in vitro was considera bly lower than that of amphotericine B used for intravenous injections and reached over 156 micrograms/ml of the medium. In mixed cultures containing fibroblasts and cells of fungi, the polyfungine derivative inhibited the growth of microbes at concentrations lower than in the medium without cells.