Comprehensive metabolomic profiling of osteosarcoma based on UHPLC-HRMS

Area of Science:

• Oncology
• Metabolomics
• Biochemistry

Background:

• Osteosarcoma (OS) is a common pediatric bone cancer.
• Tumor growth and spread are linked to metabolic reprogramming.
• Comprehensive metabolic data for OS is limited.

Purpose of the Study:

• Identify aberrant metabolic pathways in osteosarcoma.
• Discover potential adjuvant biomarkers for OS diagnosis and monitoring.

Main Methods:

• Serum samples from 65 OS patients and 30 controls.
• Nontargeted metabolomic profiling using liquid chromatography-mass spectrometry (LC-MS).
• Statistical analyses including OPLS-DA.

Main Results:

• Differential metabolites identified between primary OS, metastatic OS, and healthy controls.
• Serum adenosine-5-monophosphate, inosine-5-monophosphate, and guanosine monophosphate were elevated in primary OS.
• Lactic acid and glutamic acid showed high diagnostic potential (AUC 0.97-0.98); 5-aminopentanamide and 13(S)-HpOTrE showed metastasis monitoring potential (AUC 0.92).

Conclusions:

• New insights into osteosarcoma's metabolic landscape.
• Identified potential biomarkers for OS diagnosis and metastasis monitoring.
• Findings may have translational significance in clinical applications.