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Updated: Nov 29, 2025
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
[Multiple differentiation potential of human periodontal ligament stem cells expressing stromal cell antigen 1
Ying Zhao1, Teng Tu1, Fan Feng1
1State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Department of General Dentistry & Emergency, School of Stomatology, Air Force Medical University, Xi'an 710032, China.
Abstract:
Objective To perform in vitro characterization and comparison of stromal cell antigen 1 (STRO-1) positive (STRO-1+) and negative (STRO-1-) cell populations from human periodontal ligament cells sorted by magnetic-activated cell sorting (MACS). Methods Periodontal ligament cells were isolated from the periodontal ligament by tissue digesting method. The cells were magnetically sorted using anti-STRO-1 antibodies. Subsequently, cells were characterized by flow cytometry for the expression of CD90, CD105, CD29, STRO-1, CD34 and CD45. Colony-forming assay was used to detect the ability of colony-forming. When the cells are induced to differentiate by osteogenesis and lipogenesis, alizarin red staining, and oil red O staining were used to identify the pluripotent differentiation of STRO-1+ and STRO-1- cells. Results STRO-1+ cells highly expressed CD105 and CD90 and lowly expressed the haematopoietic markers CD45 and CD34. In contrast to STRO-1- cells, STRO-1+ cells showed a significantly higher number of colony-forming units (CFUs). STRO-1+ cells were demonstrated to have osteogenic and adipogenic differentiation potential. Conclusion STRO-1+ cells isolated by MACS are human periodontal ligament stem cells with stem cell characteristics.
