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Methods to Study Integrin Functions on Exosomes

Eiji Kawamoto1,2, Eun Jeong Park1, Motomu Shimaoka3

  • 1Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu-city, Mie, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|November 20, 2020
PubMed

Exosomes represent an important group of extracellular vesicles. They are formed in endosomal compartments and are actively secreted to extracellular spaces. Several membrane proteins, including integrins, are present on the surface of exosomes. As exosomal integrins are competent for binding to ligand, they can play important roles in directing the tissue distribution of exosomes. Integrin-directed exosomal trafficking in vivo is involved in regulating the remodeling of cell homing niches for metastatic cancers and migrating lymphocytes. This chapter describes the methods used to study integrin functions on exosomes including: isolation and biophysical characterization of exosomes, exosomal integrin-ligand binding assays, and in vivo competitive exosome homing assays.

Keywords:
Exosomal integrinExosome homing assayExosome-integrin ligand-coated beads adhesion assayV-well adhesion assay

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