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FRACTAL: Signal amplification of immunofluorescence via cyclic staining of target molecules
Yehlin Cho1, Junyoung Seo, Yeonbo Sim
1Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea. jbchang03@kaist.ac.kr.
Abstract:
In this article, we demonstrate fluorescent signal amplification via cyclic staining of target molecules (FRACTAL), a technique that can amplify the signal intensity of immunofluorescence staining more than nine-fold via simple cyclic staining of secondary antibodies. We also show that FRACTAL is compatible with four-color imaging and expansion microscopy imaging.
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