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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Nov 29, 2025

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Published on: September 28, 2016

Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretion.

David E Gordon¹, Amber S Shun-Shion², Asral W Asnawi^2,3

¹Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 22, 2020

Summary

This summary is machine-generated.

Researchers developed new quantitative flow cytometry assays to measure constitutive secretion. These novel methods are robust, insensitive to cell number variations, and ideal for high-throughput screening.

Keywords:

Assay Biosynthetic transport Constitutive secretion Flow cytometry GPI Trafficking Transport VSV-G hGH

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Last Updated: Nov 29, 2025

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

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Published on: September 28, 2016

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

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Quality-Controlled Sputum Analysis by Flow Cytometry

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Published on: August 9, 2021

Area of Science:

Cell biology
Biochemistry
Molecular biology

Background:

Constitutive secretion is a fundamental cellular process.
Traditional methods for measuring constitutive secretion, such as plate-based assays, are sensitive to cell number variations.
This sensitivity necessitates significant effort to normalize data, complicating high-throughput screening.

Purpose of the Study:

To develop novel, robust assays for measuring constitutive secretion.
To create assays that are insensitive to changes in cell number.
To facilitate functional genomic and chemical screens for studying constitutive secretion.

Main Methods:

Development of quantitative flow cytometry-based assays.
Generation of reporter cell lines specifically designed for secretion studies.
Establishment of protocols for generating and utilizing these assays.

Main Results:

A panel of quantitative flow cytometry assays for measuring constitutive secretion was successfully developed.
The developed assays demonstrate insensitivity to variations in cell number.
These assays are highly robust and suitable for large-scale screening applications.

Conclusions:

Quantitative flow cytometry assays offer a robust alternative to traditional methods for measuring constitutive secretion.
The insensitivity to cell number makes these assays ideal for functional genomic and chemical screens.
These new tools will advance the study of constitutive secretion in various biological contexts.