A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA
Kim Nguyen1, Miles Kubota1, Jon Del Arco2
1Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, California 92697, United States.
Abstract:
Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.
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