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Updated: Nov 29, 2025
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Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP
Published on: April 3, 2012
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Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral Vectors in the
Zhicheng Shao1, Ricardo Raúl Cevallos1, Kejin Hu2
1Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 23, 2020
Summary
This study details a protocol for generating induced pluripotent stem cells (iPSCs) from human fibroblasts. The method uses lentiviral vectors in a chemically defined medium for efficient iPSC production.
Area of Science:
- Stem Cell Biology
- Molecular Biology
- Biotechnology
Background:
- Induced pluripotent stem cells (iPSCs) hold significant therapeutic potential.
- Understanding iPSC reprogramming mechanisms is crucial for technological advancement.
- Current methods require optimization for efficiency and consistency.
Purpose of the Study:
- To provide a detailed protocol for generating human iPSCs.
- To optimize iPSC production using lentiviral vectors.
- To utilize chemically defined media for enhanced efficiency.
Main Methods:
- Human fibroblasts were used as starting cells.
- Lentivirus-mediated gene delivery with GFP-expressing vectors was employed.
- iPSC generation was performed in a chemically defined medium.
Main Results:
- The protocol facilitates efficient iPSC generation from fibroblasts.
- Lentiviral reprogramming in chemically defined media enhances consistency.
- The detailed protocol aids in studying human pluripotency and reprogramming.
Conclusions:
- This protocol offers a reproducible method for human iPSC generation.
- The approach leverages lentiviral vectors and chemically defined media for improved efficiency.
- This work contributes to the advancement of stem cell technology and pluripotency research.
Keywords:
Chemically defined mediumCo-expression of GFPFibroblastsHuman iPSCHuman induced pluripotent stem cellsLentiviral vectorsPluripotent reprogrammingMore Related Videos
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